Visit the nucleic acid testing laboratory to see how the new coronavirus is captured

category:Health
 Visit the nucleic acid testing laboratory to see how the new coronavirus is captured


Intimate contact with novel coronavirus specimens may be included, and personal protection must be done well. Lei Xiaonian said that after all the protection preparations are completed, the inspectors can enter the laboratory.

The first step of nucleic acid detection is sample preparation, i.e. nucleic acid extraction, which is completed in a specific safety cabinet.

A novel coronavirus suspected case of nasopharyngeal swabs was installed in the outer spiral tube of 15 ml. In order to prevent the sampling tube from happening suddenly, Lei Xiaonian wrapped three layers of plastic bags in the outer spiral tube and placed them on the vortex oscillator to perform the whirlpool oscillation, so as to mix the samples well.

This is a critical and most dangerous step, because in this process, the samples will produce aerosols, which will easily cause occupational exposure if the protection is not in place. Because of this, Lei Xiaonians operation is more cautious.

When this step is over, it means that the most dangerous nucleic acid extraction is over.

To prevent secondary pollution, two other colleagues will disinfect the test area. At this time, Lei Xiaonian also took off the three-level protective equipment, replaced the two-level protective equipment, started to configure the nucleic acid detection reaction system, and put the configured reaction system into the real-time fluorescent PCR instrument for nucleic acid amplification reaction detection. After about 3 hours, the test results will come out. For the positive samples, they will replace the test reagent, carry out repeated secondary tests, and further verify the test results. Since the outbreak, we have arranged three teams to take turns to ensure that all close contacts and suspected patients of the case can collect samples, carry out laboratory nucleic acid testing within 24 hours, and report the results in a timely manner. As of February 12, a total of 768 clinical specimens had been tested by the center, said the head of Banan CDC. Li Heng, reporter of our newspaper, source: editor in charge of peoples Daily: Zhao Changhong, Yu nh9422

To prevent secondary pollution, two other colleagues will disinfect the test area. At this time, Lei Xiaonian also took off the three-level protective equipment, replaced the two-level protective equipment, started to configure the nucleic acid detection reaction system, and put the configured reaction system into the real-time fluorescent PCR instrument for nucleic acid amplification reaction detection.

After about 3 hours, the test results will come out. For the positive samples, they will replace the test reagent, carry out repeated secondary tests, and further verify the test results.

Since the outbreak, we have arranged three teams to take turns to ensure that all close contacts and suspected patients of the case can collect samples, carry out laboratory nucleic acid testing within 24 hours, and report the results in a timely manner. As of February 12, a total of 768 clinical specimens had been tested by the center, said the head of Banan CDC. Our reporter Li Heng