Breakthrough in infertility research in China: providing basis for egg formation in vitro

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 Breakthrough in infertility research in China: providing basis for egg formation in vitro


Ovarian granulosa cells are a kind of somatic cells that interact with oocytes in follicles and promote the development of oocytes. Since the generation of oocytes, granulosa cells have been around the oocytes, supporting the growth and development of oocytes. Clinically, ovarian granulosa cells are generally discarded as a by-product of in vitro fertilization. There are thousands of granulosa cells around each egg. If we can induce them into pluripotent cells and convert them into oocytes, we can solve the problem of egg shortage. Liu Lin said.

With this idea in mind, Liu Lins team repeatedly tested and finally prepared a small molecular culture medium containing chemicals such as sodium crotonate to treat cells. This method can effectively transform ovarian granulosa cells into iPS cells, and these cells have been proved to have stable genome, gradually extended telomere and high quality.

Fine The cells successfully entered meiosis and differentiated into eggs. These eggs also have stable genomes and can further form healthy mouse offspring. Liu Lin stressed that the nature of human granulosa cells, the production of induced pluripotent stem cells and the development mode of germ cells are different from those of mice. However, recent studies have been able to use human embryonic stem cells to differentiate and form the precursor of eggs, which provides a good foundation for the formation of eggs in vitro. Recently, relevant papers were published in the international academic journal Cell reports, which was recommended as a headline article by the top international journal Cell. Source: responsible editor of China Youth Daily: Zhou Xinyi, nb12002

In addition, through another small molecule culture medium containing vitamin C and other chemicals to support the assembly of follicles and the development of eggs, the pluripotent stem cells from granulosa cells can successfully enter into meiosis and differentiate into eggs. These eggs also have stable genomes and can further form healthy mouse offspring.

Liu Lin stressed that the nature of human granulosa cells, the production of induced pluripotent stem cells and the development mode of germ cells are different from those of mice. However, recent studies have been able to use human embryonic stem cells to differentiate and form the precursor of eggs, which provides a good foundation for the formation of eggs in vitro.

Recently, relevant papers were published in the international academic journal Cell reports, which was recommended as a headline article by the top international journal Cell.